FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_80.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_80.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4086635
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG66660.16311708777514997No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG20150.017.9766731
TAGTCAG9850.016.4598089
GGTAGTC9950.016.295387
TAGGTAG10300.016.0771755
ACATAGG11000.013.80210931
TCCGCAC7350.013.61215864
CGCACCT7100.013.6053866
TATTAGG20150.013.1832162
GGCTCGG5350.012.89695553
GTCCTAA10050.012.7007891
GACTGCG5309.094947E-1212.36905840
TGGACTG10050.012.0145555
CTAATAC20250.011.9257033
GAGTAAG13000.011.67856537
GTGTTAG16650.011.6029621
GGTAGGA14000.011.58093569
GTATCCT15200.011.5750171
CGCGCAT3903.642799E-711.50105340
CCCTTAG11450.011.4491471
GTATATG28950.011.4397821
Produced by FastQC (version 0.11.2)