FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_79.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_79.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1760584
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29540.16778523489932887No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGACC3301.5643309E-1015.6814184
CTTACAC5450.014.559263
GTATTAG7850.013.6238561
TAGACCG3355.0300514E-813.3877185
TATACCG4651.0913936E-1113.3544985
TAGGTAG5053.6379788E-1212.9798675
TATTAGG7800.012.8265942
TTAGGTA7600.012.7102024
CTTAGAC4357.203198E-1012.6893323
GTACTAG5601.8189894E-1212.3211151
CCCACAC4105.478796E-811.7801873
TATACTG11500.011.6997015
GTATAGA8700.011.4997071
CTAGCCT4207.654671E-811.4997054
GTATAAC7850.011.4264591
CCCTTAG4853.9945007E-911.3811521
TATACGG6800.011.161485
TACACTA8350.011.1554045
CGCACCT3105.5638746E-511.12811666
TTATACT11850.011.0630084
Produced by FastQC (version 0.11.2)