FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_78.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_78.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3272815
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG53160.16242897933430395No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13700.016.8712831
TAGGTAG7250.015.7030175
GTATATA33050.013.8827031
TAGTCAG7400.012.585769
GCCCGAC2255.3726905E-412.26464211
ACGCGCA3408.704683E-712.17892839
TACACAG9650.012.1551075
CCTTGCG8900.012.0148719
GTATATG22500.011.8059811
GGCTCGG4156.4907E-811.6382355
ACTATGC12950.011.4540588
GGACCGT3954.3031469E-711.3537716
GTATTAT28000.011.3350561
TGACGCG3359.79739E-611.33099237
CGCGCAT4301.0585245E-711.23465540
GACGCGC3401.1549024E-511.1643638
CTCCTAG4652.6180714E-811.12838651
CTTAGAC10000.011.0395283
TAGGCCG2200.006315222510.9769714
TCCTTGC21800.010.9182568
Produced by FastQC (version 0.11.2)