FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_77.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_77.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4183362
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG69020.16498691722112502No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG17800.015.3135161
TCCGCAC6100.014.70392964
GGCTCGG6300.013.6905373
CCGCACC7200.013.41560365
TAGGTAG11650.013.029795
CGCACCT7100.012.63295366
TAGTCAG10150.012.5744569
GTATATA45900.012.328181
CTTACAC11250.012.2667223
CTATACT19950.011.9322414
GCTCGGA7000.011.8284834
GGTAGTC10550.011.7712977
CTAGGAC6750.011.75560953
CCTCCTA8600.011.6350261
TAATACT33300.011.1891064
CCGACGC4351.2486453E-711.10150769
CGCGCCG2500.00129846211.03965415
CCCTCGG5006.4610504E-911.0382028
CCCCGGC4106.9549606E-710.93972823
GTATAGG13750.010.7903011
Produced by FastQC (version 0.11.2)