FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_76.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_76.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937635
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG46490.15825655671994648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG7200.018.6862165
GTATTAG13900.016.6292061
TATTAGG13200.015.942632
CCCCCTA2152.2656002E-514.44160651
TCCGCAC5400.013.41574664
CCTTGCG8500.012.9855759
GGTAGTC7550.012.7931717
ATAGGAG8100.012.35289833
TAGTCAG7850.012.3032119
TTACGCG3651.5393744E-712.28954935
GAGCGCA3104.1483363E-612.2394289
GTATATG19050.012.1336451
GTATATA33050.011.7955571
CCTATAC10250.011.7796793
AGCGCAC4702.4465407E-911.74307410
GTATTAT27150.011.6904031
TTAGGTA10650.011.6611714
CCGCACC5652.7284841E-1111.60057865
CCCTATA9850.011.5579762
TAATACT23000.011.5492084
Produced by FastQC (version 0.11.2)