FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_75.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_75.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4201574
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG65700.15636996992079635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19750.016.4190961
TAGGTAG9650.015.0150235
CTCGGAG7600.012.7100855
TCCGCAC7500.012.4188364
CGCACCT7600.012.25542466
GTATAGA20300.012.0656531
GGTAGTC11550.011.9469267
GGCTCGG6950.011.9132565
GTATATA43300.011.8709881
TTAGGTA18000.011.6911224
GCTCGGA7500.011.4994644
CCCTAAG6700.011.327561
TAGTCAG10700.011.283589
ACTATGC17850.011.208768
GTATTAC21150.011.0914171
TATACTG27700.010.9599095
TATTAGG20800.010.9467352
GTATAAT29750.010.5521981
CTAATAC20700.010.4995113
GCCGACG28400.010.44557669
Produced by FastQC (version 0.11.2)