FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_74.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_74.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3769404
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG59450.15771724124025974No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT7250.018.08093366
CCGCACC7900.017.02992665
TCCGCAC7850.016.69917364
GTATTAG19300.015.3735461
CCCGGGG2152.2668697E-514.4409777
CTAATAC18650.013.3191033
TAGGTAG10500.013.1429255
TACTATG20950.012.6794167
ACTATGC18100.012.5785078
GTATAAT31100.011.9810881
GTATATG24950.011.75388051
TATTAGG19700.011.7338742
TAATACT31800.011.7170424
GTATATA41300.011.6952871
CTATGCA15700.011.6440859
CTAGGAC4803.3996912E-911.500063
GGCTCGG6301.8189894E-1211.500063
TATACCG10950.011.3425255
GCTCGGA6800.011.1618224
CTCGGAG6501.8189894E-1211.1462135
Produced by FastQC (version 0.11.2)