FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_73.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_73.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3587894
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG72270.20142735543469234No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGTG6050.016.53442469
GTATTAG14400.015.8118371
GGCTCGG5300.014.9710723
TAGGTAG8550.013.3152335
CTCGGAG6900.012.9994565
GTATATA34750.012.9059341
TAGTCAG8050.012.425289
GTATAGA16450.012.37334251
ACATAGG8400.012.322803531
GCTCGGA7000.012.3209134
GGGCTCG6200.012.2414232
GCGTGCA3154.9641767E-612.0444279
CCTAGAG6650.011.9318313
TATACCG10650.011.3375545
TACGCGT10100.011.270390569
TATACAG20400.011.1612985
CCTACAC6501.8189894E-1211.1456873
GTATAGG11200.011.0888211
CTTACAC8750.011.0395383
TATTATA41050.011.0092832
Produced by FastQC (version 0.11.2)