FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_72.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_72.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3077959
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG15350.018.8795531
TAGGTAG9050.018.2981175
GGTAGTC8000.017.6801727
GCTCGGA5350.016.7653316
GGCTCGG4800.016.5310485
TATTAGG17850.015.8488032
TAGTCAG8700.015.4630659
CGCACCT5100.014.88168766
TCCGCAC5700.013.92020364
ACATAGG9700.013.87344531
ATAGGAG9500.013.8018533
TTAGGTA15350.013.7099294
CTCGGAG6300.013.689647
CAGTGCG3304.141657E-813.5887549
CCGCACC5800.013.08562265
GGGCTCG6200.012.798234
ATTAGGT17250.012.5998453
GCGAAGT8850.012.47807336
CGGTCGG3104.147123E-612.23979669
TCTATAC14450.012.1763223
Produced by FastQC (version 0.11.2)