FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_71.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_71.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2432099
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG6550.018.4340865
GTATTAG10600.016.9249781
GGCTCGG3453.0559022E-1014.9988563
ACCGCGC1450.00522259414.2735988
GTATATA29000.013.9193351
GGGCTCG3751.0695658E-913.7992322
GCTCGGA3751.0695658E-913.7989474
CGCACCT4102.8194336E-1013.46294166
CTCGCGC2052.4473073E-413.461555510
AGCGGGG2851.6130998E-613.31532150
GGTAGTC5700.013.3150477
TCGCGCT1850.001728145613.05225311
GTATTAA15700.012.7455621
TAGACCG6100.011.8763875
GTCCTAG3802.6018097E-711.8029451
TCCGCAC4451.3671524E-811.62856564
CTCGGAG4451.3671524E-811.6285655
GTATAAT19450.011.5298691
ACATAGG6007.2759576E-1211.50030531
CTAATAC12350.011.4525683
Produced by FastQC (version 0.11.2)