FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_70.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_70.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2866774
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG12950.018.6473181
CGGTCGG2208.603092E-817.24666269
GGCGTCG3650.017.01634632
GGCTCGG4250.015.422433
TAGGTAG7600.014.0721035
GCTCGGA4801.8189894E-1213.6557524
TAGTCAG6450.013.369049
TTAGGTA13500.013.2884054
TATTAGG15750.013.141692
GTATTAT27400.012.59034251
GGTAGTC7150.012.5439037
TATACAG17150.012.4720665
GTGTAAG8050.012.4276861
TCCGCAC5054.7293724E-1112.29636764
GGCTAGC4802.6193447E-1012.21702652
CGCACCT4903.6925485E-1011.968533566
TATACTG20500.011.9485545
GGGCTCG5501.6370905E-1111.9171242
GGCGTCC4652.062734E-911.87115721
GTATATA34750.011.8135461
Produced by FastQC (version 0.11.2)