FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_69.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_69.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3089915
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG13300.015.8233651
CCGACGC2353.230345E-614.677169
CGCACCT5900.014.61703966
TCCGCAC5700.014.5251964
CCGCACC5850.013.56261365
TAGGTAG7600.013.1640945
GTCTTAG8000.012.9375461
ACCGTGT4701.8371793E-1012.4767478
GGCTCGG4752.2009772E-1012.34701253
TAATACT26250.012.0910814
TACACCG5753.6379788E-1211.9996545
TAGTCAG7200.011.9772699
GTATAGA14650.011.7747861
CTAATAC14650.011.5389173
GTACTAG8700.011.5000411
CCTAGGC2701.9138787E-411.4996683
GCTCGGA5401.5097612E-1011.4996684
GTATATA38500.011.470171
GTATATG20300.011.3867391
AGTCCGG5209.913492E-1011.27760710
Produced by FastQC (version 0.11.2)