FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_68.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_68.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227894
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCGG1150.001111240217.99861754
TAGGTAG5300.017.5749115
CCGGGTG1300.00252475715.9211389
GTATTAG10050.014.7617181
TAGTCAG5650.014.6530839
GCGCGTG1901.2818511E-414.52715731
CCTACAC4455.456968E-1213.9546113
CGCGCCG1500.006535661413.7986313
TAGGGGT2801.3217123E-613.553144
ACATAGG5650.013.43440731
GACCCGC1550.0081189613.35351311
CTGAGCG2851.614495E-613.3141689
GGTAGTC5750.013.1986897
CGCGTGC1850.00172551913.05481132
GTCTAGA5053.6379788E-1212.9806591
CTAGACT4852.3646862E-1112.8037164
CGCGCGT2204.4359674E-412.54646330
TATACGG8250.012.54505355
TACGGTG6700.012.3569827
TATACCG7450.012.0398855
Produced by FastQC (version 0.11.2)