FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_67.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_67.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3114734
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG7300.017.4856725
GTATTAG13400.016.4770161
GGTAGTC7500.014.718057
TAGTCAG8550.013.7157179
GGCTCGG4357.203198E-1012.6891983
CCTTGCG8450.012.65350159
GCTCGGA5257.2759576E-1212.4852646
CTAGACT6450.012.3018824
GTATATG20850.012.2441631
TCCGCAC5651.8189894E-1212.211163564
GTATAGA14350.012.0204721
GTATTAT27950.011.7258741
GGAGCGC2409.23995E-411.4977388
TCTATAC15050.011.461383
CCCTAGG2752.269602E-411.2905022
TATTAGG15600.011.27843952
CATATAC21300.011.1756533
CTCGGAG6201.2732926E-1111.1275587
TAGCGCG2802.6763827E-411.09155827
TCCTTGC17800.011.0455798
Produced by FastQC (version 0.11.2)