FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_66.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_66.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3330122
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG3700.016.7835393
GTATTAG13950.014.3440921
TCCGCAC5200.013.26843964
CTAATAC14100.013.2125733
GTATAAT27150.012.834261
GTATATA44650.012.8264331
ACCGCGC2204.4467373E-412.5431998
TATACGG13000.012.4725215
TATACAG23000.012.4494455
CCGCACC5054.7293724E-1112.29555765
ATACGGT13200.012.0223716
GGGCTCG5107.166818E-1011.5000052
CCTACAC6057.2759576E-1211.4047933
TACGGTG12100.011.4037657
GTATTAT32500.011.3584661
CTAGTAC8350.011.1555253
TAATACT28150.011.0298684
GCTCGGA5653.3469405E-1010.9908254
ACGGTGT11700.010.9082958
TATACTG19750.010.655225
Produced by FastQC (version 0.11.2)