FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_65.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_65.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2131296
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCTCGG2902.1827873E-1117.845663
GTATTAG9950.016.6443821
GCTCGGA3251.2369128E-1015.9241934
TCCGCAC3402.4556357E-1015.21915464
GGGCTCG3453.0559022E-1015.0010522
TAGGTAG5050.014.3478785
GGTAGTC4700.013.9454947
GTATAGG6550.013.6956161
CTCGGAG4053.3687684E-912.7789745
TATACAG14900.012.7360925
TATAGGA10350.012.6675552
CTAATAC10350.012.6672563
CCGCACC4104.0545274E-912.62076265
TATACTG14150.012.1919535
CTAAGAC5156.548362E-1112.0588153
CGCACCT4308.229108E-912.0337566
GCGCACT2651.6097342E-411.71532611
ACCACGC3308.315617E-611.4989178
TCTATAC11150.011.4489633
CCCTAAG3359.810599E-611.3291521
Produced by FastQC (version 0.11.2)