FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_64.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_64.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5173273
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG82350.1591835574886537No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22650.014.3185131
GGCTCGG7300.014.1780215
TAGGTAG14000.014.046375
TATTAGG26700.013.1797212
CGCACCT8250.012.96220266
GCTCGGA8700.012.6891116
GGTAGTC12350.012.2904967
CCGCACC9600.011.85806365
CTTACAC14700.011.7346443
CTAGGAC8850.011.3050373
GTATATA57450.011.2903171
TAATACT43250.011.2472434
GTATATG34250.011.1815151
GTATAGA24900.011.0848261
TAGTCAG12850.011.0057139
GTCCTAG8200.010.9395071
TCCGCAC9800.010.91248164
TATACAG33400.010.8457635
GTATTAT47850.010.7434221
CGCGGTC3602.1770169E-510.54325436
Produced by FastQC (version 0.11.2)