FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_63.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_63.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3908829
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG63650.16283649144027534No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG19500.016.2776241
CCTAGCG4251.8189894E-1214.61351630
ACCGTGC4202.7284841E-1113.9627418
TCCGCAC7450.013.89179264
GTATAGA21000.013.8005931
TAGGTAG11250.013.7998885
CGCACCT7550.013.70779666
GGCTCGG4851.8189894E-1213.5153525
TATACTG26850.013.4915095
TATTAGG20100.012.7013892
TAGTCAG9050.012.578729
CCGCACC8400.012.32053965
ACTATGC16850.012.2835078
TACACAG11750.012.0381995
TAATACT33550.011.9280624
GTATATA49000.011.8290811
CTAATAC20150.011.8133463
TATACAG24550.011.6638565
GTATAAT29450.011.5981221
TAGGACC6550.011.5873964
Produced by FastQC (version 0.11.2)