FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_62.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_62.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4887855
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79020.16166600686804336No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT8500.017.45196366
GTATTAG21650.016.413991
TAGGTAG11550.015.2340055
TCCGCAC9900.014.98385764
CCGCACC9500.014.88879365
GGCTCGG7250.014.2760865
GCTCGGA8000.014.2307456
GTATAGA21150.012.5607391
CCTCCTA9350.012.5459091
CTCGGAG8900.012.40341957
TAGTCAG11350.012.1562929
TATACGG16000.011.8595625
GGTAGTC12250.011.8275467
TAATACT39850.011.6009494
TATTAGG22950.011.5752272
CTAATAC24750.011.4302483
ATAGGAG13150.011.01986233
TCCTATA17600.010.9773342
ACATAGG12950.010.92384631
GGGCTCG9100.010.61533454
Produced by FastQC (version 0.11.2)