FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_61.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_61.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5586114
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93070.1666095607787453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24550.017.2856221
TAGGTAG12250.015.2077795
TCCGCAC10000.013.7996564
CCGCACC10500.013.79940365
CTCGGAG9300.012.9828447
TAATACT43900.012.6521864
GCTCGGA8500.012.5820346
TATACGG18050.012.4235085
GTATAGA27300.012.00583651
TATTAGG25250.011.7505132
GTATATA61500.011.6686241
CGCACCT11250.011.6528366
GGCTCGG8600.011.6334275
TAGTCAG13900.011.41575059
GTATTAT49100.011.3832151
GTCCTAC7700.011.2016171
CTAGACT12750.011.0937374
TATACCG16500.010.8724515
CTAATAC27450.010.808473
ACATAGG15100.010.73958131
Produced by FastQC (version 0.11.2)