FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_60.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_60.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3583191
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55750.155587575432066No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC3051.8189894E-1218.10102338
ACGCGCA3351.9645086E-1015.45003339
TAGGTAG7450.015.2822075
GTATTAG15850.015.019251
TCCTTGC22950.014.430078
CCTCCTA8250.013.8002971
CGCGCAT3851.5825208E-913.44372540
GTCCTAC4901.8189894E-1213.3778381
CCTTGCG10700.013.2178789
GTATAGA16450.012.3741261
TATTAGG15800.012.0100872
GTATATG23250.011.2776621
TCCTAGG4652.6142516E-811.1295822
TATACCG10250.011.1075555
CTAGACT7250.010.9450634
CCTTATA18050.010.8952752
TTATACT23900.010.82659154
CTTAGAC9250.010.8164473
TAGTCAG8750.010.6443089
GTGTAAG10100.010.5893361
Produced by FastQC (version 0.11.2)