FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_59.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_59.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5978036
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93390.1562218762148639No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24450.017.7785761
CCTCCTA12550.014.844151
TAGGTAG13500.014.3102975
GTATCCT27200.013.8249281
TCCTTGC39600.012.8923438
CGCGCGG4451.035005E-912.40295954
TAGTCAG13950.012.3636469
CGCGGGA4451.3698809E-811.62796956
GTATTAT49000.011.4761661
GGCTCGG8500.011.36405853
TATTAGG25600.011.3198582
CTTCCTA24350.011.1926631
GGTAGTC16050.011.1766547
CGCACCT9300.010.75700366
TAGACCG12550.010.7205065
GTATATA54550.010.6247771
GTATAGA25100.010.5833281
GCTCGGA10150.010.5364354
ACCGTGC8200.010.5169918
GCGACGT10850.010.4941644
Produced by FastQC (version 0.11.2)