FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_58.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_58.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5476515
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG86460.1578741225030882No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24000.015.9566821
CGCACCT9700.014.58193866
CCGCACC10200.014.20535965
TAGGTAG12650.014.1815555
TCCGCAC10250.014.13619464
GGCTCGG8450.013.0649663
TCCTTGC32750.012.8504958
TAGTCAG12850.012.617069
GGTAGTC12800.012.1280167
TATTAGG23150.012.0712712
GTATAGA25050.011.7069041
CTCGGAG10200.011.4997865
GTATTAT47250.011.4638031
GTATATG36450.011.4529861
GCTCGGA10050.011.3282524
CTAGACT10700.011.284944
ACTATGC22150.011.2132148
CCCCTAG4652.6164344E-811.1293341
CCTTGCA24850.011.1052179
CCGCGCG4703.0699084E-811.0092289
Produced by FastQC (version 0.11.2)