FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_57.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_57.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4666123
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75050.16084016645082008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG10150.015.9752555
GTATTAG20450.015.8582461
CGCGCGG3851.03682396E-1014.3358754
GGTAGTC10900.013.6085357
CGCGGGA4508.913048E-1113.03198256
TCCTTGC29450.013.001358
CCGCACC7600.012.708865
CGCACCT7900.012.66283766
GACGCGC5257.2759576E-1212.48790138
CCCCTAT8000.012.0750431
TACCTCC17200.011.8342184
CTAATAC23250.011.7224983
ACGCGCG4451.3700628E-811.62775953
CGTCCGG4207.654489E-811.50102832
CTCCTAA14400.011.5000411
TAGTCAG11300.011.2943199
TCCGCAC8250.011.28973964
GTCCTAG7350.011.2653471
TTAGGTA19050.011.2282664
CGCGCAT5552.4374458E-1011.1912740
Produced by FastQC (version 0.11.2)