FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_56.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_56.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5379839
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG90430.16809053207726105No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG12000.017.8237555
TCCGCAC9750.017.69090864
CGCACCT9750.017.3370966
GTATTAG23700.017.1776141
CCGCACC10650.016.19590265
CTAATAC26600.014.9145123
GGTAGTC14300.014.2326117
TAGTCAG12650.014.1792469
GTATAGA27400.013.7247711
TAATACT41000.013.3783344
TATTAGG25100.013.1947892
GTATATA54100.011.9891871
ACATAGG15050.011.69271631
CTTACAC15050.011.6903233
TATACTG34600.011.665375
TCCTTGC30800.011.6475638
TATACCG15650.011.4624595
TTAGGTA20950.011.3619754
CTATACT24050.011.3318494
TCTATAC25900.011.3217093
Produced by FastQC (version 0.11.2)