FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_55.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_55.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5273364
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88870.1685262007325874No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22400.018.0207651
TAGGTAG13000.015.1269175
TATTAGG23300.013.6230882
GTATTAC22600.013.4341331
GTATTAT44850.012.6158571
TAGTCAG13850.012.2029929
TAATACT39900.012.191844
TATACTG35150.012.1706925
GTATAGA23400.012.0901981
GGTAGTC13850.011.9556527
ACATAGG14050.011.78826231
GTATATG29250.011.67736151
GTCCTAG7400.011.1896091
CTAATAC24050.011.0460483
CTAGACT10500.010.8429564
GTATTAA26950.010.753651
GTCTAGA13200.010.7163111
GTATATA51000.010.6886371
TTAGGTA20700.010.6667644
ATTAGGT23350.010.6384133
Produced by FastQC (version 0.11.2)