FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_54.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_54.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5607828
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG95450.17020850140196883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG26200.016.7246461
TAGGTAG14850.015.7975795
TAGTCAG14250.015.0075999
ACATAGG15550.013.75699531
TTAGGTA22700.013.5262084
GTATAGA26700.013.43931
CCTTGCG16900.013.0625429
GGTAGTC16800.012.9362477
TATTAGG25100.012.920452
GACGCGC5650.012.82474138
TATACAG34150.012.62778955
ATTAGGT24400.012.3010263
CGCGCAT5651.8189894E-1212.21425740
CTAGACT13400.012.1005474
TATACTG34800.011.9953845
GTATTAT45550.011.8922991
ACGCGCA6100.011.8787639
TCCTTGC33800.011.6342418
CCCCCTA5351.2914825E-1011.6084241
GTATCCT24200.011.4058891
Produced by FastQC (version 0.11.2)