FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_53.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_53.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5643097
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG92110.16322597325546592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG24550.017.1449261
CGCACCT9550.015.53294566
TAGGTAG13150.013.6422385
GTATAGA26250.013.5373961
TCCGCAC11500.013.49917664
CCGCACC11400.013.31485865
TACACCG10850.012.4006045
GGTAGTC14050.012.27650457
GTCTTAG14600.012.0515971
TAATACT39600.012.0224214
GTACTAG16700.011.9822611
TATTAGG21950.011.945132
GTATATA53500.011.7366691
TATACAG30250.011.632765
TATACGG17250.011.5997045
GTCCTAA13100.011.5880031
GTATATG32250.011.4467271
CGCGCAT5855.0931703E-1111.20722740
TAGTCAG11800.011.1079169
GTATTAC24600.011.0794761
Produced by FastQC (version 0.11.2)