FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_52.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_52.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4531223
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG70980.15664645063816104No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCACCT6750.020.95363666
GTATTAG19050.017.3850021
TCCTAGG7100.015.0626652
TAGGTAG10100.014.6875835
GTATCCT19350.014.0846021
CCGCACC9600.014.01433965
TCCGCAC8900.013.95377664
GTCCTAA10400.013.9320321
CCTACAC9650.013.5849963
GTATCCC11200.013.2449061
GTATAGA19850.012.8608491
CTCCTAG7050.012.72281
TCCTTGC26850.012.5907468
GGTAGTC11250.012.572327
CGCGCAT5800.012.4931340
CGCGCGG4205.808033E-912.32057154
GTATATG27750.012.3075241
ACTATGC21550.012.16566858
TACACAG14500.012.1340415
GTCCTAG7400.012.1210451
Produced by FastQC (version 0.11.2)