FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6335037
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG98870.15606854387748642No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCGC7050.017.13062338
TAGGTAG14450.016.7123955
GTATTAG29750.016.6986221
CGCGCAT8400.014.78839140
ACGCGCA9200.014.62776339
TAGTCAG14400.014.3724939
GGTAGTC16750.013.38688857
CGCACCT12000.013.22352966
TAATACT46400.013.1602394
TATTAGG25800.012.97071552
CTAATAC31500.012.8139733
TTAGGTA23450.012.7992614
CGCGCCG4104.0599843E-912.62124415
TATACGG19700.012.60882955
ACATAGG16100.012.43003731
TGACGCG9450.012.41474937
TCCGCAC12250.012.3908564
CTCCTAG9500.012.3469711
CCCCTAG5105.638867E-1112.1760781
CCTACAC13450.012.0554683
Produced by FastQC (version 0.11.2)