FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6229610
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG93700.1504106998672469No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG27100.019.0960141
GACGCGC6100.016.97000538
TAGGTAG15550.016.1963335
TCCTTGC38050.014.9591958
ACGCGCA7400.014.45508139
CGCACCT10950.013.86206466
CGCGCAT7500.013.80227140
GTATTAT50400.013.4851711
TATTAGG26100.013.4829192
GGTAGTC16350.013.0821997
GTATCCT26500.013.0189181
GTCCTAA13650.012.6374111
TAGTCAG15600.012.3828739
TGACGCG8500.012.17837737
GCGCACT8500.012.17534411
TATACCG19700.012.08395
GTCCTAG11200.012.0134391
TCCGCAC12800.011.85827764
CCTTGCG20750.011.8031589
TATACTG39500.011.7039385
Produced by FastQC (version 0.11.2)