FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4709699
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG74230.15761092163214677No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTTGCG12500.015.1778219
GTATTAG20900.014.85590651
TAGGTAG9800.014.7850065
GACGCGC5003.6379788E-1213.11160238
TCCTTGC29250.013.0907078
TATACAG29100.012.4478585
CTCCTAG7000.012.320971
TATTAGG20050.012.2163732
CCTTGCA21800.012.18403159
TCCGCAC8050.011.999680564
CGCACCT8950.011.94927566
TATACTG30400.011.9155475
CCCTTGC14550.011.854228
CCCCGGC4451.3656972E-811.63026323
GTATATG30600.011.274091
GCGCTCG5251.1586963E-911.17255633
CTTAAGG11500.011.0994683
CTTACAC13700.011.07976153
TTAGGTA18150.011.0241484
CTCCTAT16600.011.0146491
Produced by FastQC (version 0.11.2)