FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5197253
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75330.1449419529893965No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG22500.020.5467511
TAGGTAG11950.020.2087045
TAGTCAG12700.016.2970839
TCCGCAC11100.015.22935464
TATTAGG24350.014.4513892
TATACAG31700.014.2567455
CCGCACC12250.014.08114965
TAATACT37600.014.0382184
GTATAGA24950.013.8277141
CTAATAC24600.013.1824753
CTATACA26000.013.1362134
GTATTAT40750.013.1227551
TTAGGTA18750.013.0636784
ACATAGG15250.012.67012631
GTATATA50250.012.5642321
CGCACCT12650.012.54490366
GGTAGTC16900.012.4517567
GTGTAGG8650.012.3642141
CCCCGCA8950.012.33607944
TATACTG29100.012.3295935
Produced by FastQC (version 0.11.2)