FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3803539
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57330.150728045643807No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14100.020.0643311
TAGGTAG8150.018.2014715
TATTAGG12550.016.4935682
GACGCGC5300.015.62632138
GTATTAT25400.014.8054881
CCTACAC7000.014.7853063
TGACGCG5750.014.40301137
CAGCGCA10800.014.0525799
GCGCACT7150.013.99022811
TAGTCAG8000.013.3659199
CTAATAC15350.013.260223
CGCGCAT5750.013.20276140
CTGCGCG4204.1472958E-1013.1400759
TATACCG11050.013.1124145
GTATAGA15450.012.951781
ATTATAC18850.012.81131653
TATTATA24100.012.7403122
GGTAGTC11450.012.654347
TTAGGTA13450.012.568264
TAATACT21300.012.4713214
Produced by FastQC (version 0.11.2)