FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_46.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_46.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1765920
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26000.14723203769140164No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG5850.020.052551
CCTAGCG2359.458745E-917.62163230
CTAGGAC2451.5887053E-816.8975933
GTATAGA7700.015.6828111
GGCTCGG3101.0841177E-915.5803075
TAGGTAG3351.9645086E-1015.4474265
GTCCTAG2252.0478674E-615.3343041
GTATATA11700.014.7445221
GGACCCG2605.724196E-714.5962545
CTTAAGG5050.014.3462233
CTAATAC7800.014.1535383
GTATAGG6850.014.1030821
GACGCGC2454.9562823E-614.08252338
GCTCGGA3454.9094524E-913.9985076
TACACAG5250.013.79975
CGGTCGA2001.9861072E-413.79696410
TAGTCAG3506.022674E-913.7965739
GTATTAT11300.013.4344791
CTATACT7550.013.25136854
AGACTGC4204.129106E-1013.1414556
Produced by FastQC (version 0.11.2)