FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_45.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_45.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5183049
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG79290.15297945282786252No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG12500.019.8720535
GTATTAG21500.019.0961381
TATTAGG20550.016.9565772
TAGTCAG12850.015.0323029
TTAGGTA19700.014.7108414
TATACTG29800.014.3557435
TCTATAC21450.013.8323383
GTATTAT37350.013.763621
GACGCGC5950.013.33835938
GTATAGA23550.013.331761
TATACAG25950.013.2948335
TTATACT28800.013.1772464
TAATACT32900.013.00324
GGTAGTC16500.012.9626687
TCCGCAC9400.012.84441564
ATTAGGT23500.012.7724973
ATAGGAG14650.012.71798533
CTAATAC21900.012.6028953
GTCCTAG8250.012.5459721
ATTATAC29750.012.4085563
Produced by FastQC (version 0.11.2)