FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3645425
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG51380.14094378570399885No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT4950.015.33609340
CCTCCTA7700.015.2344181
TAGGTAG6900.015.0000235
GTATCCT14250.013.80059051
GTATTAG13800.013.7505871
TCCTTGC22300.013.6123168
TAATACT22150.013.0835424
GACGCGC5400.012.77955238
CTAATAC15400.012.7696763
TGACGCG5750.012.60174937
GTATATA23550.012.5992651
CTTCCTA14050.012.5236661
CCCTCGG5601.8189894E-1212.3195888
ACTATGC15700.012.3038948
CCTTGCA17400.012.0930219
TATACAG17450.012.060195
TCCTAAG10900.012.0278722
TTAGACT14350.012.0209254
GCGACGT8650.011.96583144
CTTAAGG9250.011.9353173
Produced by FastQC (version 0.11.2)