FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2331823
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32440.13911862092448696No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG7650.021.648811
TAGGTAG4650.017.8063625
GTATAGA9750.016.278241
CTCCTAT6950.015.8861761
TATACGG6850.015.1094145
CTCCTAG3302.6193447E-914.63754751
CCTCCTA3554.6929927E-1014.5786441
CCTAATA7350.014.5515732
CCGGCCC2851.0213989E-714.5240628
CGCACCT4301.8189894E-1214.44085966
TCCGCAC4101.8189894E-1114.30327164
TCTATAC10000.014.1455363
TATTATA14900.013.66159252
TATACAG11250.013.4932665
GTATTAT14650.013.4242941
CTATACT10800.013.4171754
TATACCG6000.013.2249345
CTTACAC5750.013.2005013
GGCTCGG4457.2759576E-1113.179715
TTAGGTA6550.013.1684384
Produced by FastQC (version 0.11.2)