FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_42.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_42.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2794066
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45190.16173562113421802No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGTAG5450.018.9897175
GTATTAG9100.018.1970651
TATACAG12900.014.7084435
CCCCCTA2751.0793701E-613.7994421
TATACGG8500.013.7991945
GGCTCGG5750.013.7991945
CCCTTAA6950.012.9059521
TTAGGTA9150.012.8189244
TAATACT15950.012.761014
GTATAGA12200.012.7248941
CTAATAC10050.012.700753
GACGCGC3053.4431832E-612.44501638
CTCGGAG6950.012.4091247
GTATATA18250.012.098141
TTATACT12900.012.0341814
TAGTCAG6350.011.9512019
CCCCTAG2902.8167135E-511.896071
TCTAGAC9000.011.8826393
TCCTACA8450.011.8395442
GCTCGGA6500.011.6762416
Produced by FastQC (version 0.11.2)