FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_41.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_41.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4115501
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG61600.14967801004057588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCAC7900.014.410501564
GTATTAG15100.014.3947241
CTAATAC17300.013.7602683
CGCACCT8350.013.63388766
GTATAGA20500.012.9591531
CCTAATA16350.012.8720152
TCCTTGC21750.012.8468568
TATTAGG14400.012.6983662
TTATACT21450.012.0630714
CCGCACC9750.012.02987965
GGCTCGG6650.011.9324645
TATACTG24700.011.87260155
ACTATGC18100.011.6257888
ACCCGCG4803.3960532E-911.50096634
CTAGGAC5804.3655746E-1111.301853
CTACACT13750.011.2910344
CCTAGAC9800.011.2654313
TAATACT25300.010.9092124
TACACAG13700.010.8285885
TATACAG19350.010.6977935
Produced by FastQC (version 0.11.2)