FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_40.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_40.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5614463
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG23500.016.5888521
TATACAG31050.014.9996735
CTAATAC22200.014.4526443
TAGGTAG13150.014.1669915
CCTACAC10000.013.7999463
GTATTAT40900.013.5802681
TAATACT36850.013.5750084
TATATAC42200.013.162273
TATTATA42200.012.8352582
GTATATA38650.012.2286011
GGCTCGG7700.012.0973543
GTATAGA23700.012.0819071
TATACTG31700.011.9713485
TATTAGG23200.011.8965042
TAGTCAG13350.011.8856879
TCTATAC26050.011.78690053
ATTATAG30900.011.6116053
TATAATA40200.011.4999542
GTATAAT28950.011.2017281
TGTTAGG13300.011.154092
Produced by FastQC (version 0.11.2)