FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_39.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_39.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4442037
Sequences flagged as poor quality0
Sequence length75
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTTGC29950.016.240338
GTATCCT20900.016.0127721
GTATTAG19200.015.81331
CTAGACT8500.014.6121754
TCCGCAC8500.014.20548264
GACGCGC4403.6379788E-1214.11514738
CGCGCGG3454.9221853E-913.99771254
CCTCCTA8550.013.7199921
ACCCTTG15500.013.5771897
CCTTGCA21400.013.5401489
CTTAGAC13700.013.3470913
CGCACCT8600.013.23710466
CGCGCAT5003.6379788E-1213.11169740
TACCTCC15300.012.8533024
CCTTGCG14450.012.6521979
TCCTAGG6100.012.4429722
CCTTCTA20450.012.3160271
CCCTTGC12350.012.2901828
TAGGTAG9850.012.2592275
TAGACTG9300.012.2422795
Produced by FastQC (version 0.11.2)