FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_38.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_38.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7367051
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG75100.10194038292934308No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCCT29350.021.1589221
CGCGCCG7900.020.08838815
CCTTGCG24350.019.4082289
TCCTTGC47600.018.9143718
CGCACCG11500.018.60194836
CGCGCGG7150.017.36926354
CCGGAGC12450.017.18322240
CGTCCGG9850.017.16411632
ACGGGCC12750.017.04722221
CACGGGC13300.016.86094920
GCGCACC12800.016.71257235
GTATCCC16800.016.4289511
CGCGGGA7700.016.12903856
CCCCGCT11350.014.89350557
TCACGGG14850.014.8689319
GGATCCT17200.014.8433661
CGGGCCG14950.014.53889722
ATCCTTG60450.014.4374817
TACTCCT32050.014.4237895
GTATGCT30350.014.3232311
Produced by FastQC (version 0.11.2)