FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_37.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_37.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5304293
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG56510.10653634706830864No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG18200.019.3355081
TAGGTAG11200.015.7101015
CGCGCAT6900.015.50232340
GTATAGA23250.014.9873641
TAGTCAG10850.014.9428529
GACGCGC7600.014.52849238
TATTAGG18000.014.375122
GTATATA31350.013.9763241
TTAGGTA16050.013.3274664
TCCTATA17700.012.8645132
ACGCGCA8600.012.83901139
GGCTCGG7600.012.71075253
TATTATA29600.012.7044982
CTAATAC20300.012.5765783
GTATATG25600.012.3986581
TAATACT28600.012.3045314
CTATACT22200.012.2773614
CTCCTAA13800.012.0002141
TGACGCG9250.011.93658537
GTATTAT32900.011.8497531
Produced by FastQC (version 0.11.2)