FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_36.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_36.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3577564
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36290.1014377380809959No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG14650.020.488881
TAGGTAG7200.015.8120335
CCTCCTA6200.014.4683261
CCTTGCG11750.014.3845959
CTACCCC7350.014.0812174
GACGCGC5000.013.8032638
TTAGGTA11500.013.7995924
CGCGCAT5800.013.68445740
GTATCCT10900.013.6106411
GTCCTAG4851.8189894E-1213.5160081
ACGCGCA5150.013.4014139
TGACGCG5500.013.17565337
CTAGGGT5250.013.1424684
GCGCACT5800.013.08380711
GTATAGA13950.012.6134131
TCCTAAG10400.012.6055722
TATTAGG14100.012.4785282
GTATATA22150.012.46099951
GCGCGGC3053.4579152E-612.44051512
TCTAGAC10000.012.4196333
Produced by FastQC (version 0.11.2)