FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_35.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_35.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences471889
Sequences flagged as poor quality0
Sequence length75
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6350.13456554401564774No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG1500.032.19561
TGCCCCG450.004620650330.66572426
GCGTGGA854.4219323E-628.41089250
CTCGCCT500.00773153127.5991547
TAGGTAG1001.5614954E-524.1492565
ACATAGG1052.2739E-523.00417131
ACCTAAC750.001990418223.00173237
TCTAAGC1352.6531416E-722.9992923
TCTCGGC1052.2776321E-522.99929245
GCCTCAC1103.259752E-521.95619820
CGACGGG1103.2624215E-521.95386949
TATTAGG1906.712071E-1021.7888052
CAATCGG1304.9084592E-621.23236836
TATACTG1959.295036E-1021.2301185
ACTAGAT1154.5925794E-521.00158137
AGGCGTG1154.5963345E-520.99935348
CTAAGCA1154.5963345E-520.9993534
GGAGGTC1154.5963345E-520.99935354
TAGTCAG1154.6000918E-520.997139
GGCAGGC1507.315466E-720.69936445
Produced by FastQC (version 0.11.2)