FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_34.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_34.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3429431
Sequences flagged as poor quality0
Sequence length75
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG36770.10721895264841311No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCACC7200.016.29055865
TCCGCAC7150.015.92222564
CTAATAC18000.015.5250753
CGCACCT7450.015.28083966
GTATTAG15850.015.0196581
TCTAGAC7500.014.7200713
TATACGG13350.014.471775
TATACTG23650.014.1499695
GTATATA54200.013.7497471
CGGGTCC3102.8420982E-713.35626725
TAATACT31500.013.0333974
GACCGCG2406.376507E-512.9369967
TAGACAG10700.012.8970715
TAGGTAG9400.012.8456195
GTATAGA18200.012.7011681
CGCGCAC2457.741006E-512.67186710
ACCGCGC3002.8708782E-612.6489548
TATACCG11750.012.6254095
ATACGGT14100.012.2339236
CTAGACA7900.012.2279084
Produced by FastQC (version 0.11.2)