FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_33.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_33.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2552157
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG33430.13098723942139923No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAATAC10950.020.1645743
GTATTAG10550.019.6214221
TAGGTAG5100.018.9413555
CGCGCAT5650.018.3212740
TATACAG15650.017.8563985
TATACCG7250.017.6070615
TATTATA19150.017.2952022
TGACGCG5100.016.91424437
ATTATAG13200.016.727433
ACATAGG5800.016.65565731
GTATTAT17000.016.6416591
TACAGGC9050.016.009637
GGTGCGA7500.015.64198728
ACGCGCA5300.015.62523739
GACGCGC5300.015.62493238
TCTATAC11350.015.5023493
GTGCGAC7600.015.43586829
TCCGCAC7600.015.4337564
CGCACCT7600.015.43344866
TAATACT21250.015.4236754
Produced by FastQC (version 0.11.2)