FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_32.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_32.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2987349
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG42370.14183143650105828No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCAT4250.021.1075940
GACGCGC4600.019.50190438
TCCTTGC20800.018.7402238
CCTACAC6050.017.6767463
ACGCGCA5300.016.92589639
TGACGCG5350.016.7679937
CGTCCGG4550.016.68294732
GTATCCT13500.016.1004921
GTATTAT22400.015.5562791
GTATTAG12350.015.0854811
GCGAACC5800.014.87089523
CTTAAGG8000.014.6617213
GCGCACT5200.014.594411
CACTCCG5500.014.42578114
CCGTCCG5100.014.20726931
ATCCTTG28150.013.7255077
TATACAG13850.013.4507745
TATTATA23150.013.4122642
CTTACAC9100.013.2685253
CCCTCGG5200.013.2674148
Produced by FastQC (version 0.11.2)