FastQCFastQC Report
Fri 26 May 2017
HKFYTBGX2_n02_mini02_partii_31.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHKFYTBGX2_n02_mini02_partii_31.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4673756
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57070.12210735862120317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG16650.016.3695431
TATACAG25700.013.6920185
GTATATA43000.012.8373451
CCTATAC15600.012.8263713
TCCTTGC27150.012.4505048
ACCCTAG6400.012.3985681
TAGACTG9550.012.2821945
TCCTAGG5701.8189894E-1212.1048722
GCGCACT5451.4551915E-1112.02610611
TATTAGG18950.012.0154442
CAGCGTG8150.011.8503489
ATCCTTG40550.011.8247477
CTAGACT9350.011.8069784
GTATAGA19900.011.7890691
GTATCCT18350.011.6567981
GCTAGCC5056.0936145E-1011.6133623
GTCCTAG7150.011.5805431
TATACGG14900.011.5766845
GTGTATG16750.011.3284781
CCTTGCA20500.011.2733159
Produced by FastQC (version 0.11.2)